BLASTX 2.2.6 [Apr-09-2003]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Candida_albicans_chr7-f3-62478-63944-11
         (1464 letters)

Database: sptrembl 
           1,070,300 sequences; 339,323,595 total letters

Searching.................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

P87185|NFS1|NFS1_CANAL (SP: Candida albicans (Yeast)) Cysteine d...   856   0.0  
P87187|SPL1|NFS1_CANMA (SP: Candida maltosa (Yeast)) Cysteine de...   762   0.0  
P25374|NFS1|NFS1_YEAST (SP: Saccharomyces cerevisiae (Baker's ye...   580   e-164
Q8NIK7|5F3.050|Q8NIK7 (TrEMBL: Neurospora crassa) Probable tRNA ...   574   e-163
O60028|NFS1|NFS1_ASHGO (SP: Ashbya gossypii (Yeast) (Eremotheciu...   572   e-162
Q9HFN5||Q9HFN5 (TrEMBL: Candida rugosa (Yeast) (Candida cylindra...   567   e-160
Q96WH9||Q96WH9 (TrEMBL: Zygosaccharomyces rouxii (Candida mogii)...   555   e-157
O74351|SPBC21D10.11C|NFS1_SCHPO (SP: Schizosaccharomyces pombe (...   549   e-155
Q9VKD3|CG12264|NFS1_DROME (SP: Drosophila melanogaster (Fruit fl...   503   e-141
Q9Y697|NFS1|NFS1_HUMAN (SP: Homo sapiens (Human)) Cysteine desul...   492   e-138
Q8C6I5|NFS1|Q8C6I5 (TrEMBL: Mus musculus (Mouse)) Nitrogen fixat...   489   e-137
Q921V3|NFS1|Q921V3 (TrEMBL: Mus musculus (Mouse)) Nitrogen fixat...   489   e-137
Q99P39|NFS1|NFS1_RAT (SP: Rattus norvegicus (Rat)) Cysteine desu...   489   e-137
Q9Z1J3|NFS1|NFS1_MOUSE (SP: Mus musculus (Mouse)) Cysteine desul...   486   e-136
O61741|B0205.6|O61741 (TrEMBL: Caenorhabditis elegans) Hypotheti...   470   e-131
Q92HP1|ISCS|ISCS_RICCN (SP: Rickettsia conorii) Cysteine desulfu...   466   e-130
Q8SQS2|ECU11_1770|Q8SQS2 (TrEMBL: Encephalitozoon cuniculi) NIFS...   466   e-130
Q9ZD60|ISCS|ISCS_RICPR (SP: Rickettsia prowazekii) Cysteine desu...   466   e-130
O49543|NIFS|NFS1_ARATH (SP: Arabidopsis thaliana (Mouse-ear cres...   459   e-128
Q87S28|ISCS|ISCS_VIBPA (SP: Vibrio parahaemolyticus) Cysteine de...   436   e-121
Q93ET0||Q93ET0 (TrEMBL: Cowdria ruminantium) Hypothetical iron-s...   431   e-120
Q9KTY2|ISCS|ISCS_VIBCH (SP: Vibrio cholerae) Cysteine desulfuras...   429   e-119
Q8DEY7|ISCS|ISCS_VIBVU (SP: Vibrio vulnificus) Cysteine desulfur...   426   e-118
Q8EEU9|ISCS|Q8EEU9 (TrEMBL: Shewanella oneidensis) Cysteine desu...   425   e-118
Q8Y0M4|NIFS|Q8Y0M4 (TrEMBL: Ralstonia solanacearum (Pseudomonas ...   425   e-118
Q9JTX0|ISCS|ISCS_NEIMA (SP: Neisseria meningitidis (serogroup A)...   422   e-117
P57803|ISCS|ISCS_PASMU (SP: Pasteurella multocida) Cysteine desu...   421   e-117
Q9JYY0|ISCS|ISCS_NEIMB (SP: Neisseria meningitidis (serogroup B)...   421   e-116
P39171|ISCS|ISCS_ECOLI (SP: Shigella flexneri) Cysteine desulfur...   419   e-116
Q8ZN40|ISCS|ISCS_SALTY (SP: Salmonella typhimurium) Cysteine des...   417   e-115

>P87185|NFS1|NFS1_CANAL (SP: Candida albicans (Yeast)) Cysteine
            desulfurase, mitochondrial precursor (EC 4.4.1.-) (tRNA
            splicing protein SPL1).
          Length = 488

 Score =  856 bits (2212), Expect = 0.0
 Identities = 436/488 (89%), Positives = 436/488 (89%)
 Frame = +1

Query: 1    MYKSIFKTTGRLGKTVSSRNFVTTLPKPLATSSSPATNAPNKTSNPKTGELHVSTPVDTA 180
            MYKSIFKTTGRLGKTVSSRNFVTTLPKPLATSSSPATNAPNKTSNPKTGELHVSTPVDTA
Sbjct: 1    MYKSIFKTTGRLGKTVSSRNFVTTLPKPLATSSSPATNAPNKTSNPKTGELHVSTPVDTA 60

Query: 181  KXXXXXXXXXXXXLKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS 360
            K            LKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS
Sbjct: 61   KISIEPPEGSSISLKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS 120

Query: 361  STHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXX 540
            STHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFY        
Sbjct: 121  STHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHII 180

Query: 541  XXXXXXXCVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIG 720
                   CVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIG
Sbjct: 181  TTQTEHKCVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIG 240

Query: 721  VIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV 900
            VIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV
Sbjct: 241  VIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV 300

Query: 901  RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXX 1080
            RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQES FDKRHIE       
Sbjct: 301  RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESSFDKRHIEKLSSKLK 360

Query: 1081 XXXXXIPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS 1260
                 IPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS
Sbjct: 361  NGLLSIPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS 420

Query: 1261 YVXXXXXXXXXXXXSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDL 1440
            YV            SSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDL
Sbjct: 421  YVLHALGADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDL 480

Query: 1441 NTIEWSGH 1464
            NTIEWSGH
Sbjct: 481  NTIEWSGH 488


>P87187|SPL1|NFS1_CANMA (SP: Candida maltosa (Yeast)) Cysteine
            desulfurase, mitochondrial precursor (EC 4.4.1.-) (tRNA
            splicing protein SPL1).
          Length = 484

 Score =  762 bits (1968), Expect = 0.0
 Identities = 389/488 (79%), Positives = 412/488 (84%)
 Frame = +1

Query: 1    MYKSIFKTTGRLGKTVSSRNFVTTLPKPLATSSSPATNAPNKTSNPKTGELHVSTPVDTA 180
            MYK++ K +GR+ +    RN  +TL + LATS+S  T++   TSN +TGELHVSTP+D+ 
Sbjct: 1    MYKTVLKNSGRIQRNFIFRNVSSTLQRSLATSAS--TSSSTTTSNAETGELHVSTPLDSP 58

Query: 181  KXXXXXXXXXXXXLKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS 360
                         LKSA+RDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS
Sbjct: 59   SVHPPDGSSIS--LKSATRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHS 116

Query: 361  STHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXX 540
            STHAYGWETDKEVEKAR YIADVINADPKEIIFTSGATETNNMAIKGVPRFY        
Sbjct: 117  STHAYGWETDKEVEKARGYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHII 176

Query: 541  XXXXXXXCVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIG 720
                   CVLDSARHMQDEGF++TYLPV+SEGLINLDDLKKAIRKDTVLVS+MAVNNEIG
Sbjct: 177  TTQTEHKCVLDSARHMQDEGFDITYLPVNSEGLINLDDLKKAIRKDTVLVSVMAVNNEIG 236

Query: 721  VIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV 900
            VIQPLKEIG+ICRENK+FFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV
Sbjct: 237  VIQPLKEIGQICRENKIFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYV 296

Query: 901  RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXX 1080
            RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQES FDK+HIE       
Sbjct: 297  RRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESAFDKKHIEKLSTKLK 356

Query: 1081 XXXXXIPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS 1260
                 IPSTQFNGCN+   QYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS
Sbjct: 357  NGLLSIPSTQFNGCNNPTYQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS 416

Query: 1261 YVXXXXXXXXXXXXSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDL 1440
            YV            SSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQ GIDL
Sbjct: 417  YVLHALGADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQGGIDL 476

Query: 1441 NTIEWSGH 1464
            N+IEWSGH
Sbjct: 477  NSIEWSGH 484


>P25374|NFS1|NFS1_YEAST (SP: Saccharomyces cerevisiae (Baker's yeast))
            Cysteine desulfurase, mitochondrial precursor (EC
            4.4.1.-) (tRNA splicing protein SPL1).
          Length = 497

 Score =  580 bits (1494), Expect = e-164
 Identities = 289/415 (69%), Positives = 334/415 (80%)
 Frame = +1

Query: 220  LKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEV 399
            +  A ++ + FGTRPIYLD+QATTP DPRVLD ML+FYTGLYGNPHS+TH+YGWET+  V
Sbjct: 85   MSHAYQENTGFGTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAV 144

Query: 400  EKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSA 579
            E AR Y+A +INADPKEIIFTSGATE+NNM +KGVPRFY               CVL++A
Sbjct: 145  ENARAYVAKMINADPKEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAA 204

Query: 580  RHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICR 759
            R M  EGFEVT+L V  +GLI+L +L+ AIR DT LVS+MAVNNEIGVIQP+KEIG ICR
Sbjct: 205  RAMMKEGFEVTFLNVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICR 264

Query: 760  ENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIIT 939
            +NK++FHTDAAQAYGKI IDVNEMNIDLLSISSHKIYGPKGIGA YVRRRPRVRL+P+++
Sbjct: 265  KNKIYFHTDAAQAYGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVRRRPRVRLEPLLS 324

Query: 940  GGGQERGLRSGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNG 1119
            GGGQERGLRSGTLAPPLVAGFGEAARLMK+E   D+ HI+               T  NG
Sbjct: 325  GGGQERGLRSGTLAPPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLLSAEHTTLNG 384

Query: 1120 CNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXX 1299
              D   +YPGCVNVSFAY+EGESLLMAL+DIALSSGSACTSASLEPSYV           
Sbjct: 385  SPD--HRYPGCVNVSFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALA 442

Query: 1300 XSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
             SSIRFGIGRF+TE EVDYV++A+++RV FLR++SPLWEMVQEGIDLN+I+WSGH
Sbjct: 443  HSSIRFGIGRFSTEEEVDYVVKAVSDRVKFLRELSPLWEMVQEGIDLNSIKWSGH 497


>Q8NIK7|5F3.050|Q8NIK7 (TrEMBL: Neurospora crassa) Probable tRNA
            splicing protein spl1.
          Length = 505

 Score =  574 bits (1480), Expect = e-163
 Identities = 285/406 (70%), Positives = 324/406 (79%), Gaps = 2/406 (0%)
 Frame = +1

Query: 253  GTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVI 432
            G RPIYLD+QATTP+DPRVLD M+ ++T +YGNPHS THAYGWETDK VE+AR +IAD+I
Sbjct: 102  GQRPIYLDMQATTPIDPRVLDAMMPYFTNVYGNPHSRTHAYGWETDKAVEEARKHIADLI 161

Query: 433  NADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXX--CVLDSARHMQDEGFE 606
             ADPKEIIFTSGATE+NNM+IKGV RF+                 CVLDS RH+QDEGFE
Sbjct: 162  GADPKEIIFTSGATESNNMSIKGVARFFGRSGKKKHIITSQTEHKCVLDSCRHLQDEGFE 221

Query: 607  VTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTD 786
            VTYLPV S GLI++ +L+ AIR DT +VSIMAVNNEIGVIQPL+EIGK+CR  K+FFHTD
Sbjct: 222  VTYLPVKSSGLIDMAELEAAIRPDTAIVSIMAVNNEIGVIQPLEEIGKLCRSKKIFFHTD 281

Query: 787  AAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLR 966
            AAQA GKIP+DVN MNIDL+SISSHKIYGPKGIGACYVRRRPRVRLDPII+GGGQERGLR
Sbjct: 282  AAQAVGKIPVDVNAMNIDLMSISSHKIYGPKGIGACYVRRRPRVRLDPIISGGGQERGLR 341

Query: 967  SGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYP 1146
            SGTLAPPLV GFGEA R+ K+E  +D + I+            +  T  NG  D    YP
Sbjct: 342  SGTLAPPLVVGFGEACRIAKEEMPYDSKRIKHLSDRLLNGLLSMEHTSQNG--DPNHFYP 399

Query: 1147 GCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIG 1326
            GCVNVSFAY+EGESLLMALKDIALSSGSACTSASLEPSYV            SSIRFGIG
Sbjct: 400  GCVNVSFAYVEGESLLMALKDIALSSGSACTSASLEPSYVLRALGNSDESAHSSIRFGIG 459

Query: 1327 RFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            RFTTE E+DYV++A+ ERV FLR++SPLWE+VQEGIDLNTI+WS H
Sbjct: 460  RFTTEQEIDYVLKAVTERVGFLRELSPLWELVQEGIDLNTIQWSQH 505


>O60028|NFS1|NFS1_ASHGO (SP: Ashbya gossypii (Yeast) (Eremothecium
            gossypii)) Cysteine desulfurase, mitochondrial precursor
            (EC 4.4.1.-) (tRNA splicing protein SPL1).
          Length = 490

 Score =  572 bits (1475), Expect = e-162
 Identities = 282/415 (67%), Positives = 334/415 (80%)
 Frame = +1

Query: 220  LKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEV 399
            LK A +++   GTRPIYLD+QATTP DPRV+D ML+FYTGLYGNPHS+TH+YGWET +EV
Sbjct: 78   LKHAYQESVDHGTRPIYLDMQATTPTDPRVVDTMLKFYTGLYGNPHSNTHSYGWETSQEV 137

Query: 400  EKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSA 579
            EKAR  +ADVI ADPKEIIFTSGATE+NNMA+KGV RFY               CVL++A
Sbjct: 138  EKARKNVADVIKADPKEIIFTSGATESNNMALKGVARFYKKRKNHIITTRTEHKCVLEAA 197

Query: 580  RHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICR 759
            R M+DEGF+VT+L V+ +GL++L++L++AIR +T LVS+M+VNNEIGV+QP+KEIG ICR
Sbjct: 198  RSMKDEGFDVTFLNVNEDGLVSLEELEQAIRPETSLVSVMSVNNEIGVVQPIKEIGAICR 257

Query: 760  ENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIIT 939
             +KVFFH+DAAQAYGKIPIDV+EMNIDLLSISSHKIYGPKGIGA YVRRRPRVR++P+++
Sbjct: 258  RHKVFFHSDAAQAYGKIPIDVDEMNIDLLSISSHKIYGPKGIGALYVRRRPRVRMEPLLS 317

Query: 940  GGGQERGLRSGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNG 1119
            GGGQERG RSGTL PPLV G G AA+LM +E  +D  H+             I  T  NG
Sbjct: 318  GGGQERGFRSGTLPPPLVVGLGHAAKLMVEEYEYDSAHVRRLSDRLLKGLLSIEQTTLNG 377

Query: 1120 CNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXX 1299
               A  +YPGCVNVSFA++EGESLLMAL+DIALSSGSACTSASLEPSYV           
Sbjct: 378  --SADHRYPGCVNVSFAFVEGESLLMALRDIALSSGSACTSASLEPSYVLHAIGRDDALA 435

Query: 1300 XSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
             SSIRFGIGRFTTEAEVDYVI+AI ERV+FLR++SPLWEM ++GIDLNTIEWSGH
Sbjct: 436  HSSIRFGIGRFTTEAEVDYVIKAITERVEFLRELSPLWEMYKDGIDLNTIEWSGH 490


>Q9HFN5||Q9HFN5 (TrEMBL: Candida rugosa (Yeast) (Candida cylindracea))
            Putative SPL1 tRNA splicing protein (Fragment).
          Length = 400

 Score =  567 bits (1461), Expect = e-160
 Identities = 280/402 (69%), Positives = 321/402 (79%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RPIYLD+QATTP DPRV+DKML F  GLYGNPHSSTH+YGWET+KEVE AR +IA+VI A
Sbjct: 1    RPIYLDMQATTPTDPRVVDKMLPFSIGLYGNPHSSTHSYGWETEKEVEAARAHIANVIKA 60

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DPKEI+FTSGATE+NN AIKGV RFY               CVLD+ R +QDEGF+VTYL
Sbjct: 61   DPKEIVFTSGATESNNTAIKGVARFYGKTKRHIITTQTEHKCVLDTCRRLQDEGFDVTYL 120

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
             V+ +GLINL++L+KAIRKDT LVS+M+VNNEIGV+QPL EIG ICR+NKVFFHTD+AQA
Sbjct: 121  AVNEDGLINLEELEKAIRKDTALVSVMSVNNEIGVVQPLAEIGAICRKNKVFFHTDSAQA 180

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
            +G++ +DVN   IDL+SISSHKIYGP GIGA YVRRRPRVR++P+I GGGQERGLRSGTL
Sbjct: 181  FGRLDLDVNAQQIDLMSISSHKIYGPMGIGALYVRRRPRVRIEPLINGGGQERGLRSGTL 240

Query: 979  APPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCVN 1158
            AP L+AGFGEAARLM  E   D  HI             IP T  NG   A  +Y GCVN
Sbjct: 241  APALIAGFGEAARLMTSEYASDMAHITKLSEKLKAGLLSIPETTLNG--SAIHRYAGCVN 298

Query: 1159 VSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFTT 1338
            +SFAY+EGESLLMALKDIALSSGSACTSASLEPSYV            SSIRFGIGRFTT
Sbjct: 299  ISFAYVEGESLLMALKDIALSSGSACTSASLEPSYVLHAIGKDDALAHSSIRFGIGRFTT 358

Query: 1339 EAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            E E+DYV++AINERV++LR++SPLWEMVQEGIDL+TIEWSGH
Sbjct: 359  EEEIDYVVKAINERVEYLRELSPLWEMVQEGIDLSTIEWSGH 400


>Q96WH9||Q96WH9 (TrEMBL: Zygosaccharomyces rouxii (Candida mogii))
            NFS1-like protein (Fragment).
          Length = 387

 Score =  555 bits (1431), Expect = e-157
 Identities = 275/389 (70%), Positives = 318/389 (81%)
 Frame = +1

Query: 298  DPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATE 477
            DPRVLD ML+FYTGLYGNPHS+THAYGWET+KEVE AR  +A+VINADPKEIIFTSGATE
Sbjct: 1    DPRVLDTMLKFYTGLYGNPHSNTHAYGWETNKEVENARKNVANVINADPKEIIFTSGATE 60

Query: 478  TNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYLPVSSEGLINLDDL 657
            +NNM +KGVPRFY               CVL++AR M +EGF+V +L V +EGL+NL +L
Sbjct: 61   SNNMVLKGVPRFYKRSKKHIITTRTEHKCVLEAARAMVNEGFDVKFLNVDNEGLVNLKEL 120

Query: 658  KKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNI 837
            ++AIR DT LVS+MAVNNEIGV+QPLKEIG IC+++KV+FHTDAAQAYGK+PIDVNEMNI
Sbjct: 121  EEAIRPDTCLVSVMAVNNEIGVMQPLKEIGSICKKHKVYFHTDAAQAYGKVPIDVNEMNI 180

Query: 838  DLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAAR 1017
            DLLS+SSHKIYGPKG GA +VRRRPRVRLDPI++GGGQERGLRSGTL PPLVAGFGEAAR
Sbjct: 181  DLLSVSSHKIYGPKGAGAVFVRRRPRVRLDPILSGGGQERGLRSGTLPPPLVAGFGEAAR 240

Query: 1018 LMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLM 1197
            L+ QE+  D  HI+            IP T  NG   A+ +YPGCVNVSFAY+EGESLLM
Sbjct: 241  LVVQENDADIAHIKRLSEKLTKGLLSIPDTSLNG--SAEHRYPGCVNVSFAYVEGESLLM 298

Query: 1198 ALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFTTEAEVDYVIQAINE 1377
            A++DIALSSGSACTSASLEPSYV            SSIRFGIGRFTTE EV+YV+ AI++
Sbjct: 299  AMRDIALSSGSACTSASLEPSYVLHAIGKDDALAHSSIRFGIGRFTTEEEVEYVVDAISK 358

Query: 1378 RVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            RV FLR++SPLWEMVQEGIDLN+IEWSGH
Sbjct: 359  RVKFLRELSPLWEMVQEGIDLNSIEWSGH 387


>O74351|SPBC21D10.11C|NFS1_SCHPO (SP: Schizosaccharomyces pombe
            (Fission yeast)) Probable cysteine desulfurase,
            mitochondrial precursor (EC 4.4.1.-).
          Length = 498

 Score =  549 bits (1415), Expect = e-155
 Identities = 272/408 (66%), Positives = 315/408 (77%)
 Frame = +1

Query: 241  ASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYI 420
            +++ G  PIY+D QAT+P+D RVLD ML F+TG+YGNPHS THAYGWE +K VE AR  I
Sbjct: 93   STVTGLHPIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRTHAYGWEAEKAVENARQEI 152

Query: 421  ADVINADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEG 600
            A VINADP+EIIFTSGATE+NN  +KGV RFY               CVLDS R +Q+EG
Sbjct: 153  ASVINADPREIIFTSGATESNNAILKGVARFYKSRKKHLVSVQTEHKCVLDSLRALQEEG 212

Query: 601  FEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFH 780
            FEVT+LPV + GLINLD+L+ AIR DTV VS+MAVNNEIGV QPL+EIGKICR+ KVFFH
Sbjct: 213  FEVTFLPVQTNGLINLDELRDAIRPDTVCVSVMAVNNEIGVCQPLEEIGKICRQKKVFFH 272

Query: 781  TDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERG 960
            +DAAQ YGKI IDVN MNIDL+SIS+HKIYGPKGIGA YVRRRPRVRL+P+I+GGGQERG
Sbjct: 273  SDAAQGYGKIDIDVNRMNIDLMSISAHKIYGPKGIGAAYVRRRPRVRLEPLISGGGQERG 332

Query: 961  LRSGTLAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQ 1140
            LRSGTLAP  V GFG AAR+ K+E  +D  HI             IP T  NG  D KS+
Sbjct: 333  LRSGTLAPSQVVGFGTAARICKEEMKYDYAHISKLSQRLIDGLLAIPYTSLNG--DPKSR 390

Query: 1141 YPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFG 1320
            YPGCVN+SF Y+EGESLLM LK+IALSSGSACTSASLEPSYV            SSIRFG
Sbjct: 391  YPGCVNISFNYVEGESLLMGLKNIALSSGSACTSASLEPSYVLRAIGQSDENAHSSIRFG 450

Query: 1321 IGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            IGRFTTEAE+DY I+ ++ +V FLR MSPLW++VQEG+DL+TIEWS H
Sbjct: 451  IGRFTTEAEIDYAIENVSRQVSFLRNMSPLWDLVQEGVDLSTIEWSQH 498


>Q9VKD3|CG12264|NFS1_DROME (SP: Drosophila melanogaster (Fruit fly))
            Probable cysteine desulfurase, mitochondrial precursor
            (EC 4.4.1.-).
          Length = 462

 Score =  503 bits (1294), Expect = e-141
 Identities = 254/403 (63%), Positives = 297/403 (73%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+YLD QATTP+DPRVLD ML + T  YGNPHS THAYGWET+  VEKAR  +A +I A
Sbjct: 62   RPLYLDAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGA 121

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DPKEIIFTSGATE+NN+A+KGV RFY               CVLDS R +++EGF+VTYL
Sbjct: 122  DPKEIIFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYL 181

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV + GLI+L  L++ I  +T LVSIM VNNEIGV QP+ EIGK+CR  +VFFHTDAAQA
Sbjct: 182  PVLANGLIDLQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQA 241

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GK+P+DVN MNIDL+SIS HKIYGPKG+GA YVRRRPRVRL+PI +GGGQERGLRSGT+
Sbjct: 242  VGKVPLDVNAMNIDLMSISGHKIYGPKGVGALYVRRRPRVRLEPIQSGGGQERGLRSGTV 301

Query: 979  APPLVAGFGEAARLMKQESLFDKRHIE-XXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PL  G G AA L  +E  +DK+ ++             +P    NG  DAK+ Y GC+
Sbjct: 302  PAPLAVGLGAAAELSLREMDYDKKWVDFLSNRLLDRISSALPHVIRNG--DAKATYNGCL 359

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 360  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFT 419

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            T  EVDY      + V+ LR+MSPLWEMVQEGIDL TI+WS H
Sbjct: 420  TVEEVDYTADKCIKHVERLREMSPLWEMVQEGIDLKTIQWSQH 462


>Q9Y697|NFS1|NFS1_HUMAN (SP: Homo sapiens (Human)) Cysteine
            desulfurase, mitochondrial precursor (EC 4.4.1.-)
            (HUSSY-08).
          Length = 457

 Score =  492 bits (1267), Expect = e-138
 Identities = 243/403 (60%), Positives = 296/403 (73%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+Y+DVQATTP+DPRVLD ML +    YGNPHS THAYGWE++  +E+AR  +A +I A
Sbjct: 57   RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DP+EIIFTSGATE+NN+AIKGV RFY               CVLDS R ++ EGF+VTYL
Sbjct: 117  DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV   G+I+L +L+ AI+ DT LVS+M VNNEIGV QP+ EIG+IC   KV+FHTDAAQA
Sbjct: 177  PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DVN+M IDL+SIS HKIYGPKG+GA Y+RRRPRVR++ + +GGGQERG+RSGT+
Sbjct: 237  VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 979  APPLVAGFGEAARLMKQESLFD-KRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PLV G G A  + +QE  +D KR  +            +P    NG  D K  YPGC+
Sbjct: 297  PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNG--DPKHHYPGCI 354

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 355  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFT 414

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE EVDY ++   + V  LR+MSPLWEMVQ+GIDL +I+W+ H
Sbjct: 415  TEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457


>Q8C6I5|NFS1|Q8C6I5 (TrEMBL: Mus musculus (Mouse)) Nitrogen fixation
            gene 1.
          Length = 459

 Score =  489 bits (1260), Expect = e-137
 Identities = 243/403 (60%), Positives = 293/403 (72%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+Y+DVQATTP+DPRVLD ML +    YGNPHS THAYGWE++  +E+AR  +A +I A
Sbjct: 59   RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 118

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DP+EIIFTSGATE+NN+AIKGV RFY               CVLDS R ++ EGF VTYL
Sbjct: 119  DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 178

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV   G+I+L +L+ AI+ DT LVS+M VNNEIGV QP+ EIG+IC   KV+FHTDAAQA
Sbjct: 179  PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQA 238

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DVN+M IDL+SIS HK+YGPKG+GA Y+RRRPRVR++ + +GGGQERG+RSGT+
Sbjct: 239  VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 298

Query: 979  APPLVAGFGEAARLMKQESLFD-KRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PLV G G A  L +QE  +D KR  +            +P    NG  D K  YPGC+
Sbjct: 299  PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNG--DPKQHYPGCI 356

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 357  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFT 416

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE EVDY  +     V  LR+MSPLWEMVQ+GIDL +I+W+ H
Sbjct: 417  TEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 459


>Q921V3|NFS1|Q921V3 (TrEMBL: Mus musculus (Mouse)) Nitrogen fixation
            gene 1 (S. cerevisiae).
          Length = 459

 Score =  489 bits (1260), Expect = e-137
 Identities = 243/403 (60%), Positives = 293/403 (72%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+Y+DVQATTP+DPRVLD ML +    YGNPHS THAYGWE++  +E+AR  +A +I A
Sbjct: 59   RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 118

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DP+EIIFTSGATE+NN+AIKGV RFY               CVLDS R ++ EGF VTYL
Sbjct: 119  DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 178

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV   G+I+L +L+ AI+ DT LVS+M VNNEIGV QP+ EIG+IC   KV+FHTDAAQA
Sbjct: 179  PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQA 238

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DVN+M IDL+SIS HK+YGPKG+GA Y+RRRPRVR++ + +GGGQERG+RSGT+
Sbjct: 239  VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 298

Query: 979  APPLVAGFGEAARLMKQESLFD-KRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PLV G G A  L +QE  +D KR  +            +P    NG  D K  YPGC+
Sbjct: 299  PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQKIMKNLPDVVMNG--DPKQHYPGCI 356

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 357  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFT 416

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE EVDY  +     V  LR+MSPLWEMVQ+GIDL +I+W+ H
Sbjct: 417  TEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 459


>Q99P39|NFS1|NFS1_RAT (SP: Rattus norvegicus (Rat)) Cysteine
            desulfurase, mitochondrial precursor (EC 4.4.1.-).
          Length = 451

 Score =  489 bits (1260), Expect = e-137
 Identities = 242/403 (60%), Positives = 294/403 (72%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+Y+DV+ATTP+DPRVLD ML +    YGNPHS THAYGWE++  +E+AR  +A +I A
Sbjct: 51   RPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DP+EIIFTSGATE+NN+AIKGV RFY               CVLDS R ++ EGF VTYL
Sbjct: 111  DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV   G+I+L +L+ AI+ DT LVS+M VNNEIGV QP+ EIG+IC   K++FHTDAAQA
Sbjct: 171  PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQA 230

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DVN+M IDL+SIS HK+YGPKG+GA Y+RRRPRVR++ + +GGGQERG+RSGT+
Sbjct: 231  VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 979  APPLVAGFGEAARLMKQESLFD-KRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PLV G G A  L +QE  +D KR  +            +P    NG  D K  YPGC+
Sbjct: 291  PTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNG--DPKQHYPGCI 348

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 349  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFT 408

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE EVDY +Q     V  LR+MSPLWEMVQ+GIDL +I+W+ H
Sbjct: 409  TEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451


>Q9Z1J3|NFS1|NFS1_MOUSE (SP: Mus musculus (Mouse)) Cysteine
            desulfurase, mitochondrial precursor (EC 4.4.1.-)
            (m-Nfs1).
          Length = 451

 Score =  486 bits (1252), Expect = e-136
 Identities = 242/403 (60%), Positives = 292/403 (72%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+Y+DVQATTP+DPRVLD ML +    YGNPHS THAYGWE++  +E+AR  +A +I A
Sbjct: 51   RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
            DP+EIIFTSGATE+NN+AIKGV RFY               CVLDS R ++ EGF VTYL
Sbjct: 111  DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV   G+I+L +L+ AI+ DT LVS+M VNNEIGV QP+ EI +IC   KV+FHTDAAQA
Sbjct: 171  PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIRQICSSRKVYFHTDAAQA 230

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DVN+M IDL+SIS HK+YGPKG+GA Y+RRRPRVR++ + +GGGQERG+RSGT+
Sbjct: 231  VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 979  APPLVAGFGEAARLMKQESLFD-KRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
              PLV G G A  L +QE  +D KR  +            +P    NG  D K  YPGC+
Sbjct: 291  PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQKIMKNLPDVVMNG--DPKQHYPGCI 348

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLMALKD+ALSSGSACTSASLEPSYV            SSIRFGIGRFT
Sbjct: 349  NLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVFRAIGTDEDLAHSSIRFGIGRFT 408

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE EVDY  +     V  LR+MSPLWEMVQ+GIDL +I+W+ H
Sbjct: 409  TEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451


>O61741|B0205.6|O61741 (TrEMBL: Caenorhabditis elegans) Hypothetical
            protein.
          Length = 412

 Score =  470 bits (1209), Expect = e-131
 Identities = 235/404 (58%), Positives = 297/404 (73%), Gaps = 2/404 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            +PIYLDVQAT P+DPRV+D ML +    +GNPHS TH+YGW+ ++ VE+AR ++A++I A
Sbjct: 11   QPIYLDVQATAPMDPRVVDAMLPYMINDFGNPHSRTHSYGWKAEEGVEQAREHVANLIKA 70

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXX-CVLDSARHMQDEGFEVTY 615
            DP++IIFTSGATE+NN+AIKGV +F                 CVLDS R++++EGF+VTY
Sbjct: 71   DPRDIIFTSGATESNNLAIKGVAKFRKQSGKNHIITLQTEHKCVLDSCRYLENEGFKVTY 130

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            LPV   G+++++ L ++I  +T LVSIM VNNEIGV+QP+K+IG++CR   V+FHTDAAQ
Sbjct: 131  LPVDKGGMVDMEQLTQSITAETCLVSIMFVNNEIGVMQPIKQIGELCRSKGVYFHTDAAQ 190

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            A GK+PIDVNEM IDL+SIS+HKIYGPKG GA YVRRRPRVR++  ++GGGQERGLRSGT
Sbjct: 191  ATGKVPIDVNEMKIDLMSISAHKIYGPKGAGALYVRRRPRVRIEAQMSGGGQERGLRSGT 250

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIE-XXXXXXXXXXXXIPSTQFNGCNDAKSQYPGC 1152
            +A PL  G GEAA++  +E   DK H+E             +P    NG  DA+  YPGC
Sbjct: 251  VAAPLCIGLGEAAKIADKEMAMDKAHVERLSQMLINGISDKLPHIIRNG--DARHAYPGC 308

Query: 1153 VNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRF 1332
            VN+SFAY+EGESLLMALK IALSSGSACTSASLEPSYV            SSIRFG+GRF
Sbjct: 309  VNLSFAYVEGESLLMALKSIALSSGSACTSASLEPSYVLRAIGSEEDLAHSSIRFGLGRF 368

Query: 1333 TTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TT+ EV + I       + LR +SPLWEMVQEGIDL +I+W+ H
Sbjct: 369  TTDEEVKHTIDLCIRETNRLRDLSPLWEMVQEGIDLKSIQWTQH 412


>Q92HP1|ISCS|ISCS_RICCN (SP: Rickettsia conorii) Cysteine desulfurase
            (EC 4.4.1.-).
          Length = 410

 Score =  466 bits (1200), Expect = e-130
 Identities = 227/404 (56%), Positives = 289/404 (71%), Gaps = 1/404 (0%)
 Frame = +1

Query: 256  TRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            T PIY+D QATTP+DPRV++ ML ++T  +GNPHS +H++GWE +  VE+AR+ +A +I 
Sbjct: 9    TLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIG 68

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            AD KEIIFTSGATE+NN+AIKG+ +FY               CVLD+ RH++ EG ++TY
Sbjct: 69   ADTKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITY 128

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            LP+   G+I+L+ LK AI   T+LVS+M VNNEIGV+QPLKEIGKICRE  VFFH+D AQ
Sbjct: 129  LPIKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGKICREKGVFFHSDIAQ 188

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
             +GKIPIDVN  NIDL SIS HKIYGPKGIGA YVR++PRVR+ P+I GGGQERG+RSGT
Sbjct: 189  GFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQERGMRSGT 248

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIE-XXXXXXXXXXXXIPSTQFNGCNDAKSQYPGC 1152
            L  PL+ G G AA +   E   D +H+              I     NG  D   +Y G 
Sbjct: 249  LPTPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNG--DKNQRYKGN 306

Query: 1153 VNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRF 1332
            +N+SFA +EGES+++A+KD+A+SSGSACTSASLEPSYV            ++IRFGIGRF
Sbjct: 307  LNLSFAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRF 366

Query: 1333 TTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TTE EVDY +  I  ++D LR++SPLWEM+QEGIDL  I+W+ H
Sbjct: 367  TTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH 410


>Q8SQS2|ECU11_1770|Q8SQS2 (TrEMBL: Encephalitozoon cuniculi) NIFS-like
            protein (Cysteine DESULFURASE) involved in iron-sulfur
            cluster synthesis.
          Length = 432

 Score =  466 bits (1199), Expect = e-130
 Identities = 240/405 (59%), Positives = 293/405 (72%), Gaps = 3/405 (0%)
 Frame = +1

Query: 253  GTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVI 432
            G + I+LDVQ+TTPVDPRVLD ML FYT ++GNPHS TH YGW+ +  VEKAR+ +A +I
Sbjct: 30   GGKRIFLDVQSTTPVDPRVLDAMLPFYTTVFGNPHSRTHRYGWQAEAAVEKARSQVASLI 89

Query: 433  NADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXX--CVLDSARHMQDEGFE 606
              DPKEIIFTSGATE+NN+A+KGV  F                  C+LD+ R++++ G E
Sbjct: 90   GCDPKEIIFTSGATESNNLALKGVSGFKLKEGKAAHIITLQTEHKCILDTCRNLEENGVE 149

Query: 607  VTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTD 786
            VTYLPV ++G++++DD+KK+I+++TVLVSI AVN+EIG +QPLKEIG +C+E  V FHTD
Sbjct: 150  VTYLPVGNDGVVDIDDVKKSIKENTVLVSIGAVNSEIGTVQPLKEIGMLCKERGVLFHTD 209

Query: 787  AAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLR 966
            AAQ  GKI IDVNEMNIDLLS+ +HKIYGPKGIGA YVRRRPRVR+ P+I GGGQERGLR
Sbjct: 210  AAQGVGKIQIDVNEMNIDLLSMCAHKIYGPKGIGALYVRRRPRVRMVPLINGGGQERGLR 269

Query: 967  SGTLAPPLVAGFGEAARLMKQESLFDKRHI-EXXXXXXXXXXXXIPSTQFNGCNDAKSQY 1143
            SGT+A PLV GFG+AA +  +E   D  HI E            I     NG   ++  +
Sbjct: 270  SGTVASPLVVGFGKAAEICSKEMKRDFEHIKELSKKLKNMFKKNIEGVIING---SEKGF 326

Query: 1144 PGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGI 1323
            PGCVNVSF ++EGESLLM LKDIALSSGSACTSASLEPSYV            SSIRFGI
Sbjct: 327  PGCVNVSFPFVEGESLLMHLKDIALSSGSACTSASLEPSYVLRALGRDDELAHSSIRFGI 386

Query: 1324 GRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWS 1458
            GRFT   E+D V     E V  LR+MSPL+EMV+EGIDL+ I W+
Sbjct: 387  GRFTMAKEIDIVANKTVEAVQKLREMSPLYEMVKEGIDLSKISWT 431


>Q9ZD60|ISCS|ISCS_RICPR (SP: Rickettsia prowazekii) Cysteine
            desulfurase (EC 4.4.1.-) (NifS protein homolog).
          Length = 410

 Score =  466 bits (1199), Expect = e-130
 Identities = 224/404 (55%), Positives = 289/404 (71%), Gaps = 1/404 (0%)
 Frame = +1

Query: 256  TRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            T PIY+D Q+TTP+DPRV++ ML ++T  +GNPHS +H++GWE +  VE AR+ +A VI 
Sbjct: 9    TLPIYMDYQSTTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVENARSMVAKVIG 68

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            AD KEIIFTSGATE+NN+ IKG+ +FY               CVL++ RH++ EG ++TY
Sbjct: 69   ADSKEIIFTSGATESNNLVIKGIAKFYGNKKKHIITLVSEHKCVLNACRHLEQEGIKITY 128

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            LP+ S G+I+L+ LK AI   T+LVS+MAVNNEIGVIQPLKEIGKICRE  VFFH+D AQ
Sbjct: 129  LPIKSNGIIDLETLKNAITDQTLLVSVMAVNNEIGVIQPLKEIGKICRERNVFFHSDIAQ 188

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
             +GKIPI+VNE NIDL SIS HKIYGPKGIGA Y+R++PRVR+ P+I GGGQERG+RSGT
Sbjct: 189  GFGKIPINVNECNIDLASISGHKIYGPKGIGALYIRKKPRVRVTPLINGGGQERGMRSGT 248

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIE-XXXXXXXXXXXXIPSTQFNGCNDAKSQYPGC 1152
            L  PL+ G G A+ +   E   D +H+              I     NG  D   +Y G 
Sbjct: 249  LPTPLIVGLGIASEIAYNEMEKDTQHVNYLFDRFLNNIHSKISEVYLNG--DKDQRYKGN 306

Query: 1153 VNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRF 1332
            +N+SFA +EGES+++A+KD+A+SSGSACTSASLEPSYV            +SIRFGIGRF
Sbjct: 307  LNLSFAGVEGESIILAIKDLAVSSGSACTSASLEPSYVLRSIGISEELAHTSIRFGIGRF 366

Query: 1333 TTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TTE E+DY +  +  ++D LR++SPLWEM+QEG+DL  I W+ H
Sbjct: 367  TTEQEIDYAVNLVCSKIDKLRRLSPLWEMMQEGVDLKKIRWTAH 410


>O49543|NIFS|NFS1_ARATH (SP: Arabidopsis thaliana (Mouse-ear cress))
            Cysteine desulfurase, mitochondrial precursor (EC
            4.4.1.-).
          Length = 453

 Score =  459 bits (1180), Expect = e-128
 Identities = 230/403 (57%), Positives = 289/403 (71%), Gaps = 1/403 (0%)
 Frame = +1

Query: 259  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 438
            RP+YLD+QATTP+DPRV D M       YGNPHS TH YGWE +  VE AR  +A +I A
Sbjct: 53   RPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEA 112

Query: 439  DPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYL 618
             PKEI+F SGATE NNMA+KGV  FY               CVLDS RH+Q EGFEVTYL
Sbjct: 113  SPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYL 172

Query: 619  PVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQA 798
            PV ++GL++L+ L++AIR DT LVSIMAVNNEIGV+QP++EIG IC+E+ V FHTDAAQA
Sbjct: 173  PVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQA 232

Query: 799  YGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTL 978
             GKIP+DV + N+ L+S+S+HKIYGPKG+GA YVRRRPR+RL+P++ GGGQERGLRSGT 
Sbjct: 233  IGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTG 292

Query: 979  APPLVAGFGEAARLMKQESLFDKRHIE-XXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            A   + GFG A  L  +E  +D++ I+             +     NG  D  S+Y G +
Sbjct: 293  ATQQIVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMD--SRYVGNL 350

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFAY+EGESLLM LK++A+SSGSACTSASLEPSYV            +SIRFGIGRFT
Sbjct: 351  NLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFT 410

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            T+ E+D  ++   ++V+ LR+MSPL+EMV+EGID+  I+WS H
Sbjct: 411  TKEEIDKAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453


>Q87S28|ISCS|ISCS_VIBPA (SP: Vibrio parahaemolyticus) Cysteine
            desulfurase (EC 4.4.1.-).
          Length = 404

 Score =  436 bits (1121), Expect = e-121
 Identities = 219/403 (54%), Positives = 279/403 (69%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  AT PVDPRV +KM+++ T  G +GNP S +H YGW+ ++ V+ AR  IAD++N
Sbjct: 4    PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIADLLN 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            ADP+EI+FTSGATE++N+AIKG   FY                VLD  R ++ EGFEVTY
Sbjct: 64   ADPREIVFTSGATESDNLAIKGAAHFYSKKGKHVITCKTEHKAVLDPCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L   S GLI+L+ LK A+R DTVLVSIM VNNEIGVIQ +  IG++CRE K+ FH DAAQ
Sbjct: 124  LEPESNGLIDLEKLKAAMRDDTVLVSIMHVNNEIGVIQDIAAIGELCRERKIVFHVDAAQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+PIDV EM +DL+S+S+HK+YGPKGIGA YVRR+PR+RL+  + GGG ERG RSGT
Sbjct: 184  SAGKLPIDVQEMKVDLISLSAHKVYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            LA   + G GEA R+ K+E   D  H              + +   NG  D + + P  +
Sbjct: 244  LATHQIVGMGEAFRIAKEEMQKDYDHALALRNRLLNGVKDLEAVTVNG--DLEQRVPQNL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFA++EGESLLM+LKD+A+SSGSACTSASLEPSYV            SS+RF  GRFT
Sbjct: 302  NISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGMDDELAHSSVRFSFGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+DY I+ I   V  LR MSPLW+M +EG+DL+T+EW+ H
Sbjct: 362  TEEEIDYAIEQIRVAVTKLRDMSPLWDMYKEGVDLSTVEWAHH 404


>Q93ET0||Q93ET0 (TrEMBL: Cowdria ruminantium) Hypothetical iron-sulfur
            cofactor synthesis protein 2.
          Length = 413

 Score =  431 bits (1109), Expect = e-120
 Identities = 227/404 (56%), Positives = 281/404 (69%), Gaps = 3/404 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINAD 441
            PI+LD Q+TT  D RVLD M+ ++   + NPHS +H +GW+ +  VE AR  IA +INA+
Sbjct: 14   PIFLDYQSTTKTDSRVLDAMIPYFQE-FSNPHSRSHCFGWKAESAVELARERIASLINAE 72

Query: 442  PKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYLP 621
             KE+IFTSGATE+NN+AIKGV  FY               CVLDS RH++ EGF+VTYL 
Sbjct: 73   SKEVIFTSGATESNNLAIKGVANFYKSKGNHIITVRTEHKCVLDSCRHLETEGFDVTYLD 132

Query: 622  VSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAY 801
            V   G+++L+ LK AI   T+LVS+M VNNEIGVIQP++EIGKIC  + VFFHTDAAQA+
Sbjct: 133  VQKNGILDLNLLKSAITDKTILVSVMMVNNEIGVIQPIEEIGKICHNHGVFFHTDAAQAF 192

Query: 802  GKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRR-PRVRLDPIITGGGQERGLRSGTL 978
            GKIPIDVN+MNIDLLSIS HKIY P GIGA YVR+R PR+RL PII GGGQERG+RSGT+
Sbjct: 193  GKIPIDVNKMNIDLLSISGHKIYAPMGIGALYVRKRQPRIRLTPIINGGGQERGMRSGTI 252

Query: 979  APPLVAGFGEAARLMKQESLFDK--RHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGC 1152
              PL  G GEAA  + QE + D+  R  E            +P    NG  D + +  G 
Sbjct: 253  PTPLAVGLGEAA-YIAQEVMEDEAIRIKELRDILYSEIKKHLPYVILNG--DYEQRIAGN 309

Query: 1153 VNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRF 1332
            +N+SF Y+EGES++MA+ ++A+SSGSACTSASLEPSYV            SSIRFGIGRF
Sbjct: 310  LNLSFPYVEGESIIMAINNLAVSSGSACTSASLEPSYVLRALNIDKDLEHSSIRFGIGRF 369

Query: 1333 TTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TT+AE+ Y    I   +  LR+MSPLWEMVQEGI+LN I+W  H
Sbjct: 370  TTKAEILYAADLIVNSIKRLREMSPLWEMVQEGINLNEIKWDVH 413


>Q9KTY2|ISCS|ISCS_VIBCH (SP: Vibrio cholerae) Cysteine desulfurase (EC
            4.4.1.-) (NifS protein homolog).
          Length = 404

 Score =  429 bits (1103), Expect = e-119
 Identities = 218/403 (54%), Positives = 276/403 (68%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  AT PVDPRV +KM+++ T  G +GNP S +H YGW+ ++ V+ AR  IA ++N
Sbjct: 4    PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLN 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            ADP+EI+FTSGATE++N+AIKGV  FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L   S GL++L  L+ A+R DT+LVSIM VNNEIGV+Q +  IG++CR  KV FH DAAQ
Sbjct: 124  LDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVDAAQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+ IDV EM +DL+S+S+HK YGPKGIGA YVRR+PR+RL+  + GGG ERG RSGT
Sbjct: 184  SAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA R+ K+E   D  H              + +   NG  D   + P  +
Sbjct: 244  LPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTING--DLDQRVPHNL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            NVSFA++EGESLLMALKD+A+SSGSACTSASLEPSYV            SSIRF  GRFT
Sbjct: 302  NVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TEAE+DY I+ I   VD LR MSPLW+M ++G+DLNT+EW+ H
Sbjct: 362  TEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH 404


>Q8DEY7|ISCS|ISCS_VIBVU (SP: Vibrio vulnificus) Cysteine desulfurase
            (EC 4.4.1.-).
          Length = 404

 Score =  426 bits (1095), Expect = e-118
 Identities = 214/403 (53%), Positives = 277/403 (68%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  AT PVDPRV +KM+++ T  G +GNP S +H YGW+ ++ V+ AR  IA+++N
Sbjct: 4    PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAELLN 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            ADP+EI+FTSGATE++N+AIKG   FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADPREIVFTSGATESDNLAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L   S GLI+L  L+ A+R DTVLVSIM VNNEIGVIQ ++ IG++CR  K+ FH DAAQ
Sbjct: 124  LEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVIQDIEAIGELCRSRKIIFHVDAAQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+ IDV ++ +DL+S+S+HKIYGPKGIGA YVRR+PR+RL+  + GGG ERG RSGT
Sbjct: 184  SAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA R+ K +   D +H              + +   NG  D   + P  +
Sbjct: 244  LPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTING--DLDQRVPHNL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SFA++EGESLLM+LKD+A+SSGSACTSASLEPSYV            SSIRF  GRFT
Sbjct: 302  NISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+DY I+ I   V+ LR MSPLW+M ++GIDLNT+EW+ H
Sbjct: 362  TEEEIDYAIEQIRVAVEKLRDMSPLWDMYKDGIDLNTVEWAHH 404


>Q8EEU9|ISCS|Q8EEU9 (TrEMBL: Shewanella oneidensis) Cysteine
            desulfurase.
          Length = 404

 Score =  425 bits (1092), Expect = e-118
 Identities = 215/403 (53%), Positives = 273/403 (67%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  ATTPVDPRV +KM ++ T  G++GNP S +H YGW+ ++ V+ AR  +AD+IN
Sbjct: 4    PIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARNQVADLIN 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            AD +EI+FTSGATE+NN+AIKGV  FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADHREIVFTSGATESNNLAIKGVAHFYKKKGKHIITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L   S G+I +  L+ A+R DT+LVSIM VNNEIGVI  +  IG++CR   + FH DAAQ
Sbjct: 124  LEPESNGIIPMARLEAAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMDAAQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+PIDV    +DL+SIS HK+YGPKGIGA YVRR+PR+RL+  + GGG ERG+RSGT
Sbjct: 184  SAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA+ + K E   D   I             I  T  NG  D   +  G +
Sbjct: 244  LPTHQIVGLGEASAIAKAEMATDNVRIAKLRDKLWNGIKHIEETYVNG--DMNQRTSGSL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            NVSF Y+EGESL+MALKD+A+SSGSACTSASLEPSYV            SSIRF IGRFT
Sbjct: 302  NVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+D+ I+ I + +D LR+MSPLWEM ++GIDLN ++W+ H
Sbjct: 362  TEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH 404


>Q8Y0M4|NIFS|Q8Y0M4 (TrEMBL: Ralstonia solanacearum (Pseudomonas
            solanacearum)) Probable pyridoxal-phosphate-dependent
            aminotransferase (NIFS protein) (EC 2.6.1.-).
          Length = 405

 Score =  425 bits (1092), Expect = e-118
 Identities = 201/401 (50%), Positives = 279/401 (69%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINAD 441
            PIY+D  ATTPVDPRV+DKM+ +    +GNP S +HA+GWE +K VE AR  +A ++NAD
Sbjct: 7    PIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVAALVNAD 66

Query: 442  PKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYLP 621
            P+EI++TSGATE++N+A+KG   FY                VLD+AR ++ +GFEVTYL 
Sbjct: 67   PREIVWTSGATESDNLALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGFEVTYLD 126

Query: 622  VSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAY 801
            V   GLI+++  K+A+R DT+L S+M+VNNEIGVIQ +++IG+ICR+  V FH DAAQA 
Sbjct: 127  VQENGLIDIEQFKQALRPDTILASVMSVNNEIGVIQDIEQIGEICRDKGVIFHVDAAQAT 186

Query: 802  GKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTLA 981
            GK+ +D+ ++ +DL+S S+HK YGPKGIGA YVRR+PR+R++  + GGG ERG+RSGTL 
Sbjct: 187  GKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGMRSGTLP 246

Query: 982  PPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCVNV 1161
               + G GEA R+ ++E   +   I             +     NG  D + + P  +N+
Sbjct: 247  THQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNG--DMERRVPHNLNI 304

Query: 1162 SFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFTTE 1341
            SF ++EGESL+MA+KD+A+SSGSACTSASLEPSYV            SSIRF +GRFTTE
Sbjct: 305  SFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTE 364

Query: 1342 AEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
             EVDYV++ +  ++  LR +SPLWEM ++G+DLN+I+W+ H
Sbjct: 365  QEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSIQWAAH 405


>Q9JTX0|ISCS|ISCS_NEIMA (SP: Neisseria meningitidis (serogroup A))
            Cysteine desulfurase (EC 4.4.1.-) (NifS protein homolog).
          Length = 404

 Score =  422 bits (1085), Expect = e-117
 Identities = 212/401 (52%), Positives = 272/401 (67%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINAD 441
            P+YLD  ATT VD RV +KM+ + T  +GNP S++HA+GWE ++ VEKAR  IA +INAD
Sbjct: 6    PVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINAD 65

Query: 442  PKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYLP 621
            PKEI+FTSGATE++N+AIKG   FY                VLD+ R ++ +GFEVTYL 
Sbjct: 66   PKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFEVTYLG 125

Query: 622  VSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAY 801
            V   GLI+L++LK AIR DT+LVS+M  NNEIGV+Q +  IG+ICRE K+ FH DAAQA 
Sbjct: 126  VQENGLIDLEELKAAIRDDTILVSVMWANNEIGVVQDIPAIGEICRERKIVFHVDAAQAC 185

Query: 802  GKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTLA 981
            GK+P+DV    IDLLS+S+HK+YGPKGIGA YVRR+PRVRL+  + GGG ERG RSGTL 
Sbjct: 186  GKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFRSGTLP 245

Query: 982  PPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCVNV 1161
               + G GEA R+ K+E   D  H              I     NG  D + + P  +NV
Sbjct: 246  THQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYING--DLEHRVPNNLNV 303

Query: 1162 SFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFTTE 1341
            SF ++EGESL+MA+K++A+SSGSACTSASLEPSYV            SS+R   GR TTE
Sbjct: 304  SFNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTE 363

Query: 1342 AEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
             EV +  + I  ++  LR++SPLWEM ++GIDLN+IEW+ H
Sbjct: 364  EEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH 404


>P57803|ISCS|ISCS_PASMU (SP: Pasteurella multocida) Cysteine
            desulfurase (EC 4.4.1.-).
          Length = 404

 Score =  421 bits (1083), Expect = e-117
 Identities = 213/403 (52%), Positives = 277/403 (68%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  AT PVD RV  KM+E+ T  G +GNP S +H +GW+ ++ V+ AR YIAD+I 
Sbjct: 4    PIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIADLIG 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            AD +EI+FTSGATE++N+AIKG   FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADSREIVFTSGATESDNLAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L   S+GLI+L++LK A+R DT+LVSIM VNNEIGVIQ +  IG++CR  K+ FH DA Q
Sbjct: 124  LNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVIQDIAAIGELCRARKILFHVDATQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+PI++ E+ +DL+S+SSHK+YGPKGIGA YV R+PRVRL+ II GGG ERG+RSGT
Sbjct: 184  SVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA R+ K+E   +   ++            I  T  NG    + +    +
Sbjct: 244  LPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNG--SMEHRLDSNL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            N+SF Y+EGESL+MAL+DIA+SSGSACTSASLEPSYV            SSIRF +GR+T
Sbjct: 302  NISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+DY I+ +   V  LR++SPLW+M +EGIDLNTIEW+ H
Sbjct: 362  TEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIEWTHH 404


>Q9JYY0|ISCS|ISCS_NEIMB (SP: Neisseria meningitidis (serogroup B))
            Cysteine desulfurase (EC 4.4.1.-) (NifS protein homolog).
          Length = 404

 Score =  421 bits (1081), Expect = e-116
 Identities = 210/401 (52%), Positives = 271/401 (67%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINAD 441
            P+YLD  AT PVD RV +KM+ + T  +GNP S++H++GWE ++ VEKAR  IA +INAD
Sbjct: 6    PVYLDYAATPPVDKRVAEKMIPYLTETFGNPASNSHSFGWEAEEAVEKARADIAALINAD 65

Query: 442  PKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTYLP 621
             KEI+FTSGATE+NN+AIKG   FY                VLD+ R ++ +G+EVTYL 
Sbjct: 66   SKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKTEHKAVLDTMRELERQGYEVTYLD 125

Query: 622  VSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAY 801
            V   GL++LD LK AIR+DT+LVS+M VNNEIGV+Q +  IG+ICRE K+ FH DAAQA 
Sbjct: 126  VQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVVQDIPAIGEICRERKIIFHVDAAQAC 185

Query: 802  GKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTLA 981
            GK+P+DV    +DLLS+S HK+YGPKGIGA YVRR+PRVRL+  + GGG ERG RSGTL 
Sbjct: 186  GKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFRSGTLP 245

Query: 982  PPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCVNV 1161
               + G GEA R+ K+E   D  H              I     NG  D + + P  +NV
Sbjct: 246  THQIVGMGEAFRIAKEELAQDTAHYLKLRDIFLKGIEGIEEVYING--DLEHRVPNNLNV 303

Query: 1162 SFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFTTE 1341
            SF ++EGESL+MA+K++A+SSGSACTSASLEPSYV            SS+R   GR TTE
Sbjct: 304  SFNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTE 363

Query: 1342 AEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
             EV +  + I  ++  LR++SPLWEM ++GIDLN+IEW+ H
Sbjct: 364  EEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH 404


>P39171|ISCS|ISCS_ECOLI (SP: Shigella flexneri) Cysteine desulfurase
            (EC 4.4.1.-) (ThiI transpersulfidase) (NifS protein
            homolog).
          Length = 404

 Score =  419 bits (1078), Expect = e-116
 Identities = 206/403 (51%), Positives = 274/403 (67%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  ATTPVDPRV +KM++F T  G +GNP S +H +GW+ ++ V+ AR  IAD++ 
Sbjct: 4    PIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVG 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            ADP+EI+FTSGATE++N+AIKG   FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L     G+I+L +L+ A+R DT+LVSIM VNNEIGV+Q +  IG++CR   + +H DA Q
Sbjct: 124  LAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+PID++++ +DL+S S HKIYGPKGIGA YVRR+PRVR++  + GGG ERG+RSGT
Sbjct: 184  SVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA R+ K+E   +   +             I     NG  D +   P  +
Sbjct: 244  LPVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNG--DLEHGAPNIL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            NVSF Y+EGESL+MALKD+A+SSGSACTSASLEPSYV            SSIRF +GRFT
Sbjct: 302  NVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSLGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+DY I+ + + +  LR +SPLWEM ++G+DLN+IEW+ H
Sbjct: 362  TEEEIDYTIELVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 404


>Q8ZN40|ISCS|ISCS_SALTY (SP: Salmonella typhimurium) Cysteine
            desulfurase (EC 4.4.1.-) (ThiI transpersulfidase) (NifS
            protein homolog).
          Length = 404

 Score =  417 bits (1071), Expect = e-115
 Identities = 204/403 (50%), Positives = 274/403 (67%), Gaps = 2/403 (0%)
 Frame = +1

Query: 262  PIYLDVQATTPVDPRVLDKMLEFYT--GLYGNPHSSTHAYGWETDKEVEKARTYIADVIN 435
            PIYLD  ATTPVDPRV +KM++F T  G +GNP S +H +GW+ ++ V+ AR  IA+++ 
Sbjct: 4    PIYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAELVG 63

Query: 436  ADPKEIIFTSGATETNNMAIKGVPRFYXXXXXXXXXXXXXXXCVLDSARHMQDEGFEVTY 615
            ADP+EI+FTSGATE++N+AIKG   FY                VLD+ R ++ EGFEVTY
Sbjct: 64   ADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 616  LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 795
            L     G+I+L++L+ A+R DT+LVSIM VNNEIGV+Q +  IG++CR   + +H DA Q
Sbjct: 124  LAPQRNGIIDLNELEAAMRDDTILVSIMHVNNEIGVVQDIATIGEMCRARGIIYHVDATQ 183

Query: 796  AYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLRSGT 975
            + GK+PID++++ +DL+S S HKIYGPKGIGA YVRR+PR+R++  + GGG ERG+RSGT
Sbjct: 184  SVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRIRIEAQMHGGGHERGMRSGT 243

Query: 976  LAPPLVAGFGEAARLMKQESLFDKRHIEXXXXXXXXXXXXIPSTQFNGCNDAKSQYPGCV 1155
            L    + G GEA R+ K+E   +   +             I     NG  D +   P  +
Sbjct: 244  LPVHQIVGMGEAYRIAKEEMETEMARLRGLRNRLWNGIKDIEEVYLNG--DLEQGAPNIL 301

Query: 1156 NVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVXXXXXXXXXXXXSSIRFGIGRFT 1335
            NVSF Y+EGESL+MALKD+A+SSGSACTSASLEPSYV            SSIRF +GRFT
Sbjct: 302  NVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRFSLGRFT 361

Query: 1336 TEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSGH 1464
            TE E+DY I  + + +  LR +SPLWEM ++G+DLN+IEW+ H
Sbjct: 362  TEEEIDYTIDLVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 404


  Database: sptrembl
    Posted date:  Oct 14, 2003  6:47 PM
  Number of letters in database: 339,323,595
  Number of sequences in database:  1,070,300
  
Lambda     K      H
   0.317    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 434,420,963
Number of Sequences: 1070300
Number of extensions: 9036161
Number of successful extensions: 30525
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 27748
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29586
length of database: 339,323,595
effective HSP length: 125
effective length of database: 205,536,095
effective search space used: 74404066390
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)